Summary
The human amniotic membrane contains the cellular parts obligatory for cell integration
and tissue remodeling and has high tensile strength and persistence.
We theorized that such material could function as a scaffold to improve the survival
of ischemic tissue in random skin flaps. Male Albino Wistar rats (n = 30) were randomly assigned to three groups, each receiving a different model of
AM on dorsal paravertebral areas: saline, amniotic membrane sheet, and micronized
amniotic membrane. Digital photographs were taken, and the survival area was examined
after one week. Histological analysis of skin flap tissue was performed, and the expression
rate of vascular endothelial growth factor and apoptotic protein was examined. The
survival percentage increased over time in all groups; however, one week after the
implanted AM was increased survival in both experimental groups, with significantly
greater than in the group control. In the experimental groups, there was a more regular
arrangement of collagen and improved epithelialization in the flap tissue, also an
increased number of inflammatory cells was observed in the control group. Additionally,
VEGF and apoptotic protein expression, respectively, were significantly lower and
greater in the control group than in the experimental groups. These results show that
micronized membrane is an excellent scaffold for promoting flap survival.
Keywords
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Article info
Publication history
Published online: August 23, 2022
Accepted:
August 16,
2022
Received:
March 28,
2022
Identification
Copyright
© 2022 British Association of Plastic, Reconstructive and Aesthetic Surgeons. Published by Elsevier Ltd. All rights reserved.
