Summary
Background
High-intensity focused ultrasound (HIFU) therapy has emerged as an option for skin
rejuvenation. However, the application against photo-damaged skin remains obscure.
This study evaluates the effect of HIFU against photoaged skin using a mouse model.
Methods
A total of 60 mice were used and divided into 3 groups; group 1: natural aging control
group (n = 20), group 2: UVB irradiation group (n = 20), and group 3: UVB irradiation followed by HIFU treatment (n = 20). The evaluation was made grossly by analyzing wrinkles and histologically by
performing H&E, Toluidine Blue, Masson's Trichrome, and immunohistochemistry for TGF-β
and MMP3. Imaging software was used to quantify the findings.
Results
Gross findings showed HIFU treated group 3 with similar findings with the control
group supporting the rejuvenation effect for photo-aged skin. Histology findings with
H&E show a significant reduction in skin thickness after HIFU treatment (60.115 units
(group 2) vs. 40.853 units (group 3), p<0.05). Toluidine Blue and Masson's Trichrome showed improved collagen array and significantly
increased distribution for group 3 over group 2 (272,879.88 units (group 2) vs. 533,805.78
units (group 3), p<0.05). Immunohistochemistry for TGF-β showed a significantly higher value for group
3 (2.45450 units) over group 2 (0.58880 units) and MMP3 with a significantly lower
value for group 3 (99,180 units) over group 2 (559,830 units) (p<0.05).
Conclusions
The treatment of HIFU supports the rejuvenation effect for photoaged skin. Findings
show that HIFU provides benefits of collagen formation and rearrangement by enhancing
TGF-β and inhibiting MMP3 activity. This study is the first animal study to show the
direct effect of HIFU on photo-aged skin further supporting the use of HIFU in aging
skin aiming to reverse the morphological effects of aging.
Keywords
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References
- Comparison of the effectiveness of ablative and non-ablative fractional laser treatments for early stage thyroidectomy scars.Arch Plast Surg. 2016; 43: 575-581
- The efficacy and safety of ablative fractional resurfacing using a 2,940-Nm Er:YAG laser for traumatic scars in the early posttraumatic period.Arch Plast Surg. 2012; 39: 232-237
- A pilot study of skin resurfacing using the 2,790-nm Erbium:YSGG laser system.Arch Plast Surg. 2015; 42: 52-58
- A review of the use of ultrasound for skin tightening, body contouring, and cellulite reduction in dermatology.Dermatol Surg. 2018; 44: 949-963
- Intense focused ultrasound tightening in Asian skin: clinical and pathologic results.Dermatol Surg. 2011; 37: 1595-1602
- Long-term efficacy of micro-focused ultrasound with visualization for lifting and tightening lax facial and neck skin using a customized vectoring treatment method.J Clin Aesthet Dermatol. 2016; 9: 27-33
- Efficacy of high-intensity focused ultrasound in facial and neck rejuvenation.J Cosmet Dermatol. 2020; 19: 353-358
- High-intensity focused ultrasound: a review of mechanisms and clinical applications.Ann Biomed Eng. 2021; 49: 1975-1991
- High-intensity focused ultrasound: principles, therapy guidance, simulations and applications.Z Med Phys. 2012; 22: 311-322
- A portable high-intensity focused ultrasound device for noninvasive venous ablation.J Vasc Surg. 2010; 51: 707-711
- Therapeutic applications of ultrasound.Prog Biophys Mol Biol. 2007; 93: 111-129
- Intense focused ultrasound (IFUS) with a modified parameter on facial tightening: a study on its safety and efficacy.J Cosmet Laser Ther. 2016; 18: 448-451
- High-intensity focused ultrasound therapy: an overview for radiologists.Korean J Radiol. 2008; 9: 291-302
- Randomized sham-controlled trial to evaluate the safety and effectiveness of a high-intensity focused ultrasound device for noninvasive body sculpting.Plast Reconstr Surg. 2011; 128: 253-262
- Evaluation of a novel high-intensity focused ultrasound device: preclinical studies in a porcine model.Aesthet Surg J. 2011; 31: 429-434
- High-intensity focused ultrasound for the treatment of wrinkles and skin laxity in seven different facial areas.Ann Dermatol. 2015; 27: 688-693
- The effect of high and low ultraviolet-B dose exposure on the degree of hairless mouse skin wrinkling.Photochem Photobiol. 1991; 53: 109-112
- Chronological ageing and photoageing of the fibroblasts and the dermal connective tissue.Clin Exp Dermatol. 2001; 26: 592-599
- Elastin changes during chronological and photo-ageing: the important role of lysozyme.J Eur Acad Dermatol Venereol. 2006; 20: 980-987
- Rejuvenation of facial skin and improvement in the dermal architecture by transplantation of autologous stromal vascular fraction: a clinical study.Bioimpacts. 2016; 6: 149-154
- Collagen extracts derived from young and aged mice demonstrate different structural properties and cellular effects in three-dimensional gels.J Gerontol A Biol Sci Med Sci. 2010; 65: 209-218
- Collagen fibers, reticular fibers and elastic fibers. A comprehensive understanding from a morphological viewpoint.Arch Histol Cytol. 2002; 65: 109-126
- Assessment of safety and efficacy of a bipolar fractionated radiofrequency device in the treatment of photodamaged skin.J Cosmet Laser Ther. 2012; 14: 208-211
- Fighting against skin aging: the way from bench to bedside.Cell Transplant. 2018; 27: 729-738
- Ulthera: initial and six month results.Facial Plast Surg Clin North Am. 2012; 20 (vi): 163-176
- High intensity focused ultrasound: physical principles and devices.Int J Hyperthermia. 2007; 23: 89-104
- Elderly and sun-affected skin. Distinguishing between changes caused by aging and changes caused by habitual exposure to sun.Can Fam Physician. 2001; 47: 1236-1243
- Molecular mechanisms of skin aging: state of the art.Ann NY Acad Sci. 2007; 1119: 40-50
- Transforming growth factor-beta 1-induced collagen production in cultures of cardiac fibroblasts is the result of the appearance of myofibroblasts.Methods Find Exp Clin Pharmacol. 2002; 24: 333-344
- Transforming growth factor beta 1 increases collagen content, and stimulates procollagen I and tissue inhibitor of metalloproteinase-1 production of dental pulp cells: role of MEK/ERK and activin receptor-like kinase-5/Smad signaling.J Formos Med Assoc. 2017; 116: 351-358
- Structural studies of the MMP-3 interaction with triple-helical collagen introduce new roles for the enzyme in tissue remodelling.Sci Rep. 2019; 9: 18785
- Role of matrix metalloproteinases in photoaging and photocarcinogenesis.Int J Mol Sci. 2016; : 17
- Pathophysiological mechanisms of high-intensity focused ultrasound-mediated vascular occlusion and relevance to non-invasive fetal surgery.J R Soc Interface. 2014; 1120140029
- Noninvasive skin tightening: focus on new ultrasound techniques.Clin Cosmet Investig Dermatol. 2015; 8: 47-52
- High intensity focused ultrasound-induced gene activation in sublethally injured tumor cells in vitro.J Acoust Soc Am. 2005; 118: 3328-3336
- Matrix-degrading metalloproteinases in photoaging.J Investig Dermatol Symp Proc. 2009; 14: 20-24
- Inhibition effect of Gynura procumbens extract on UV-B-induced matrix-metalloproteinase expression in human dermal fibroblasts.J Ethnopharmacol. 2011; 137: 427-433
Article info
Publication history
Published online: June 23, 2022
Accepted:
June 6,
2022
Received:
April 15,
2022
Footnotes
#The authors in this manuscript does not have any conflict of issues.
Identification
Copyright
© 2022 British Association of Plastic, Reconstructive and Aesthetic Surgeons. Published by Elsevier Ltd. All rights reserved.
