Summary
The effect of differentiated and undifferentiated adipose-derived stem cells on the
repair of peripheral nerve gaps was studied. Adipose-derived stem cells were maintained
in differentiation medium for 2 weeks. The expression of Schwann cell proteins S-100,
nerve growth factor receptor (NGFR) p75 and integrin β4 was examined by immunofluorescence
staining and real time-polymerase chain reaction (real time-PCR) at the end of the
differentiation period. A 10-mm gap on the left sciatic nerves of 20 Fischer rats
was created and bridged with silicone tube (group I), silicone tube filled with collagen
gel (group II), nerve graft (group III), silicone tube filled with adipose-derived
stem cells (group IV) and silicone tube filled with differentiated adipose-derived
stem cells (group V). In vitro, the positivity of differentiated adipose-derived stem cells for S-100, NGFR p75
and integrin β4 by immunofluorescence staining was 31%, 27% and 12%, respectively.
Fold changes by real time-PCR in comparison with undifferentiated cells were 48.4,
168.7 and 284.85, respectively. In vivo, a walking track analysis did not yield any statistically significant differences
after 3 months postoperatively; however, after 6 months, group IV (sciatic function
index (SFI) = −49.1 ± 13.1) and V (SFI = −52.6 ± 5.7) showed significant improvement
compared to other groups (I: −73.3 ± 5.07, II: −79.6 ± 12.01, III: −74.8 ± 12.89)
(p < 0.05). Nerve conduction velocity after 6 months was higher in groups IV (4.44 ± 0.3 mm ms−1), V (4.25 ± 0.3 mm ms−1) and III (4 ± 0.3 mm ms−1) than in groups I (2.5 ± 2.25 mm ms−1) and II (2.35 ± 1.58 mm ms−1) (p > 0.05). Myelin fibre density and myelinated fibre/unmyelinated fibre ratio were
significantly higher in the midnerve and the distal nerve in groups IV and V (p < 0.05). These results reveal the therapeutic potential of adipose-derived stem cells
in nerve reconstruction.
Keywords
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Article info
Publication history
Published online: December 05, 2011
Accepted:
November 10,
2011
Received:
May 22,
2011
Footnotes
☆This work was performed in the Laboratory for Stem Cell Biology and Tissue Regeneration, Nippon Medical School, Tokyo, Japan.
☆This article was previously presented as an oral presentation at the Plastic Surgery Research Council Meeting, Pittsburgh, Pennsylvania, 2009 and at the World Society for Reconstructive Microsurgery Meeting, Okinawa, Japan, 2009.
Identification
Copyright
© 2011 British Association of Plastic, Reconstructive and Aesthetic Surgeons. Published by Elsevier Inc. All rights reserved.
