Summary
Traumatic injuries resulting in peripheral nerve lesions often require a graft to
bridge the gap. Although autologous nerve auto-graft is still the first-choice strategy
in reconstructions, it has the severe disadvantage of the sacrifice of a functional
nerve. Cell transplantation in a bioartificial conduit is an alternative strategy
to create a favourable environment for nerve regeneration. We decided to test new
fibrin nerve conduits seeded with various cell types (primary Schwann cells and adult
stem cells differentiated to a Schwann cell-like phenotype) for repair of sciatic
nerve injury.
Two weeks after implantation, the conduits were removed and examined by immunohistochemistry
for axonal regeneration (evaluated by PGP 9.5 expression) and Schwann cell presence
(detected by S100 expression). The results show a significant increase in axonal regeneration
in the group of fibrin seeded with Schwann cells compared with the empty fibrin conduit.
Differentiated adipose-derived stem cells also enhanced regeneration distance in a
similar manner to differentiated bone marrow mesenchymal stem cells. These observations
suggest that adipose-derived stem cells may provide an effective cell population,
without the limitations of the donor-site morbidity associated with isolation of Schwann
cells, and could be a clinically translatable route towards new methods to enhance
peripheral nerve repair.
Keywords
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References
- Tissue engineered nerve constructs: where do we stand?.J Cell Mol Med. 2006; 10: 309-317
- Repair of extended peripheral nerve lesions in rhesus monkeys using acellular allogenic nerve grafts implanted with autologous mesenchymal stem cells.Exp Neurol. 2007; 204: 658-666
- Skin-derived stem cells transplanted into resorbable guides provide functional nerve regeneration after sciatic nerve resection.Glia. 2007; 55: 425-438
- Adipose-derived stem cells differentiate into a Schwann cell phenotype and promote neurite outgrowth in vitro.Exp Neurol. 2007; 207: 267-274
- Alternative techniques for peripheral nerve repair: conduits and end-to-side neurorrhaphy.Acta Neurochir Suppl. 2007; 100: 43-50
- The peripheral nerve allograft in the primate immunosuppressed with Cyclosporin A: I. Histologic and electrophysiologic assessment.Plast Reconstr Surg. 1992; 90: 1036-1046
- Composite PHB-GGF conduit for long nerve gap repair: a long-term evaluation.Scand J Plast Reconstr Surg Hand Surg. 2005; 39: 129-137
- New fibrin conduit for peripheral nerve repair.J Reconstr Microsurg. 2009; 25: 27-33
- Fibrin matrix for suspension of regenerative cells in an artificial nerve conduit.J Plast Reconstr Aesthet Surg. 2008; 61: 669-675
- Structural parameters of collagen nerve grafts influence peripheral nerve regeneration.Biomaterials. 2005; 26: 3083-3091
- Peripheral nerve regeneration and neurotrophic factors.J Anat. 1999; 194: 1-14
- Nerve guides seeded with autologous schwann cells improve nerve regeneration.Exp Neurol. 2000; 161: 571-584
- Retroviral labeling of Schwann cells: in vitro characterization and in vivo transplantation to improve peripheral nerve regeneration.Glia. 2001; 34: 8-17
- Bone marrow stromal cells and resorbable collagen guidance tubes enhance sciatic nerve regeneration in mice.Exp Neurol. 2006; 198: 457-468
- Transplantation of bone marrow stromal cell-derived Schwann cells promotes axonal regeneration and functional recovery after complete transection of adult rat spinal cord.J Neuropathol Exp Neurol. 2005; 64: 37-45
- Engraftment and migration of human bone marrow stromal cells implanted in the brains of albino rats–similarities to astrocyte grafts.Proc Natl Acad Sci U S A. 1998; 95: 3908-3913
- Stem-cell plasticity and therapy for injuries of the peripheral nervous system.Biotechnol Appl Biochem. 2004; 40: 17-24
- Sciatic nerve regeneration in rats induced by transplantation of in vitro differentiated bone-marrow stromal cells.Eur J Neurosci. 2001; 14: 1771-1776
- Green fluorescent protein is a stable morphological marker for schwann cell transplants in bioengineered nerve conduits.Tissue Eng. 2004; 10: 1359-1367
- Transdifferentiation of mesenchymal stem cells into Schwann cell-like myelinating cells.Eur J Cell Biol. 2006; 85: 11-24
- The potential of adipose-derived adult stem cells as a source of neuronal progenitor cells.Plast Reconstr Surg. 2005; 116: 1453-1460
- From heterogeneity to plasticity in adipose tissues: site-specific differences.Exp Cell Res. 2006; 312: 727-736
- Stem cell therapy for neurologic disorders: therapeutic potential of adipose-derived stem cells.Curr Drug Targets. 2005; 6: 57-62
- Multilineage cells from human adipose tissue: implications for cell-based therapies.Tissue Eng. 2001; 7: 211-228
- Multipotential differentiation of adipose tissue-derived stem cells.Keio J Med. 2005; 54: 132-141
- Clonal analysis of the differentiation potential of human adipose-derived adult stem cells.J Cell Physiol. 2006; 206: 229-237
- Concise review: adipose tissue-derived stromal cells–basic and clinical implications for novel cell-based therapies.Stem Cells. 2007; 25: 818-827
- Fat tissue: an underappreciated source of stem cells for biotechnology.Trends Biotechnol. 2006; 24: 150-154
- Adult bone marrow stromal cells differentiate into neural cells in vitro.Exp Neurol. 2000; 164: 247-256
- Neurogenic differentiation of murine and human adipose-derived stromal cells.Biochem Biophys Res Commun. 2002; 294: 371-379
- In vitro differentiation of human processed lipoaspirate cells into early neural progenitors.Plast Reconstr Surg. 2003; 111: 1922-1931
- Characterization of neuronal/glial differentiation of murine adipose-derived adult stromal cells.Exp Neurol. 2004; 187: 319-328
- The differentiation of rat adipose-derived stem cells into OEC-like cells on collagen scaffolds by co-culturing with OECs.Neurosci Lett. 2007; 421: 191-196
- Phenotypic and functional characteristics of mesenchymal stem cells differentiated along a Schwann cell lineage.Glia. 2006; 54: 840-849
- Rat bone marrow mesenchymal stem cells express glial markers and stimulate nerve regeneration.Neurosci Lett. 2004; 362: 200-203
- Mesothelial cell sheets cultured on fibrin gel prevent adhesion formation in an intestinal hernia model.Tissue Eng. 2005; 11: 618-625
- A biodegradable fibrin scaffold for mesenchymal stem cell transplantation.Biomaterials. 2003; 24: 2497-2502
- Comparative analysis of mesenchymal stem cells from bone marrow, umbilical cord blood, or adipose tissue.Stem Cells. 2006; 24: 1294-1301
- Adipose-derived stem cells for regenerative medicine.Circ Res. 2007; 100: 1249-1260
- Human adipose tissue is a source of multipotent stem cells.Mol Biol Cell. 2002; 13: 4279-4295
- Adipose stromal cells-conditional medium protected glutamate-induced CGNs neuronal death by BDNF.Neurosci Lett. 2009; 452: 238-240
- Secretion of angiogenic and antiapoptotic factors by human adipose stromal cells.Circulation. 2004; 109: 1292-1298
- Mesenchymal stem cells from rat visceral fat exhibit multipotential differentiation in vitro.Anat Rec A Discov Mol Cell Evol Biol. 2003; 272: 398-402
- Mature adipocyte-derived dedifferentiated fat cells exhibit multilineage potential.J Cell Physiol. 2008; 215: 210-222
- Bone marrow stromal damage after chemo/radiotherapy: occurrence, consequences and possibilities of treatment.Leuk Lymphoma. 2001; 42: 863-870
Article info
Publication history
Published online: October 15, 2009
Accepted:
September 10,
2009
Received:
March 30,
2009
Identification
Copyright
© 2009 British Association of Plastic, Reconstructive and Aesthetic Surgeons. Published by Elsevier Inc. All rights reserved.
