Introduction: Malignant melanoma causes more than three quarters of all skin cancer deaths. Current
assessment and treatment is complicated by the lack of a reliable non-invasive pre-operative
assessment technique. A key feature of melanoma invasion and spread is collagen re-modelling
by Matrixmetalloproteinases. Thus collagen architecture can be used as a reliable
marker of Melanoma invasion through the tissues, and to define melanoma invasion borders
Second Harmonic Generation (SHG) imaging can reliably demonstrates collagen architecture
in human skin. SHG imaging employs infrared or longer spectrum pulsed lasers, which
enables good depth penetration and no phototoxicity to tissues. Our aims were to use
SHG to define collagen architecture in unstained histological sections thereby demonstrating
melanoma borders, and to prove that SHG is an interchangeable with conventional techniques
such as Melan-A and H&E.
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© 2007 Published by Elsevier Inc.
