Summary
Objective
To study and explore the feasibility of using candidate epidermal stem cells with
reconstruct tissue-engineered skin for a skin defect.
Methods
After the candidate epidermal stem cells were selected directly by rapid adhesion
to type IV collagen within 10 min from keratinocytes isolated from foreskin epidermis, the TES was constructed by
seeding large-scale cultured candidate epidermal stem cells onto a fibroblast-containing
dermal substrate, then grafted onto athymic immunodeficient mice with full-thickness
skin defects. All specimens were harvested after 1 week, 2 weeks and 4 weeks of transplantation
to evaluate by gross, histological, transmission electron microscopic and immunohistochemical
techniques its potential to reconstitute a full-thickness skin defect.
Results
The transplanted skin developed a well-differentiated epidermis composed of stratum
basale, prickle cell layer, granular layer and stratum corneum and clearly defined
dermis with the morphological features of intact skin. The continuous and integral
basement membrane zone (BMZ) was established; hemidesmosomes, basal lamina and anchoring
fibrils were detected. In the dermis, the collagen of dermal substitute degraded gradually
and fibroblasts were aligned in order; lymphocytes, organelle debris, differentiated
microvasculature and hyperactive collagen fibrillogenesis were observed. The immunohistochemistry
suggested that the keratinocytes of the TES were originated from the human candidate
epidermal stem cells and not from the mice.
The constructed TES was similar to the uninjured skin in morphological features, which
suggested the constructed TES by combining cultured candidate epidermal stem cells
with the dermal substrate could satisfy the need for the restoration of skin defects.
Keywords
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Article info
Publication history
Published online: July 10, 2006
Accepted:
December 9,
2005
Received:
January 31,
2005
Identification
Copyright
© 2006 British Association of Plastic, Reconstructive and Aesthetic Surgeons. Published by Elsevier Inc. All rights reserved.
