Summary
In the course of development of a new type of filler for the correction of small defects
in soft tissues we studied macroporous gelatine spheres as culture substrate, transplantation
vehicle, and biodegradable scaffold for guided regeneration of soft tissues in vivo.
We injected intradermally in nude mice gelatine spheres that had either been preseeded
with human fibroblasts or preadipocytes, or left unseeded. We compared the extent
of regenerated tissue with that found after injections of saline or single-cell suspensions
of human fibroblasts or preadipocytes. Routine histological examinations and immunohistochemical
staining for von Willebrand factor (indicating neoangiogenesis) were made after 7,
21, and 56 days. Injected saline or single-cell suspensions had no effect. However,
a quick and thorough tissue regeneration with developing neoangiogenesis was elicited
by the gelatine spheres and the effect of spheres preseeded with preadipocytes surpassed
the effect of spheres preseeded with fibroblasts, which in turn surpassed the effect
of unseeded gelatine spheres. We suggest that minor soft tissue defects such as wrinkles
or creases can be corrected by injection of naked macroporous gelatine spheres, whereas
larger defects are best corrected by injection of macroporous gelatine spheres preseeded
with fibroblasts, or preadipocytes, or both.
Keywords
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Article info
Publication history
Published online: May 28, 2006
Accepted:
October 16,
2005
Received:
May 11,
2005
Identification
Copyright
© 2006 The British Association of Plastic Surgeons. Published by Elsevier Inc. All rights reserved.
