Summary
Background
Reperfusion injury is a phenomenon that occurs when tissues are subjected to ischaemia
for a variable period of time, after which they are reperfused. Many factors have
been implicated in the cause of reperfusion injury including free radicals and neutrophils.
Caffeic acid (3,4-dihydroxycinnamic acid) phenethyl ester (CAPE) is an active component
of propolis from honeybee; it has anti-inflammatory and immunomodulatory properties,
and protective effects against ischaemia-reperfusion (I/R) injury. We investigated
the effects of CAPE on the survival of skin flaps in the rat.
Materials and methods
Eighteen Wistar rats were used, and randomly divided into three groups (n=6 rats each group): the control group (Group 1), ethanol group (Group 2), and CAPE
group (Group 3). A caudally based rectangular flap, 3×10 cm in size, was elevated on the back of the rat, according to the method described
by Khouri and colleagues. The flap was sutured back into its original place. In the
control group, saline 0.2 ml/day was given intraperitoneally (i.p.). Five percent ethanol 0.2 ml/day was administered i.p. in the ethanol group, and CAPE 50 μmol/kg/day i.p. in the CAPE group. To observe the effects of CAPE, levels of malondialdehyde
(MDA), nitric oxide (NO), glutathione (GSH), glutathione peroxidase (GSH-Px), and
superoxide dismutase (SOD) were measured from extracted skin tissue. Flap viability
was evaluated seven days after the initial operation, measuring necrotic areas of
flaps and total flap areas.
Results
MDA and NO levels were significantly decreased in CAPE group; and however, GSH, GSH-Px,
and SOD enzyme activities were significantly increased in CAPE group.
We believed that the CAPE had beneficial effects to improve the survival of skin flaps
since it has antioxidative and anti-inflammatory properties, and protective effects
against I/R injury.
Keywords
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Article info
Publication history
Published online: May 24, 2006
Accepted:
January 24,
2006
Received:
July 15,
2004
Identification
Copyright
© 2006 The British Association of Plastic Surgeons. Published by Elsevier Inc. All rights reserved.
