Summary
Effective blockade of the pluripotent cytokine transforming growth factor (TGF)-β
as a means of cutaneous scar reduction is a strategy with great potential. This desired
effect may be achieved through the overexpression of mutant TGF-β receptors within
the wound milieu. Our goal was to examine the effects of dominant negative mutant
TGF-β receptor II (TGFβRIIdn) protein expression in a well-established rabbit ear
model of hypertrophic scarring. Serial injections of a retroviral construct encoding
a truncated TGFβRII and the marker green fusion protein (pMSCV-rIIdn-GFP) were performed
in 7 mm punch wounds at day 10 and day 12 (two-day injection group) or days 8, 10, 12 (three-day
injection group) post-wounding. Delivery of an empty vector (pMSCV-GFP) at the same
time points served as a negative control. Histomorphometric analysis of wounds harvested
at day 28 revealed a modest, though statistically significant reduction (20%, p=0.027) in the scar elevation index (SEI) in two-day treated and a more modest reduction
in SEI (12%) in the three-day treated arm compared to null-treated controls. Confocal
microscopy confirmed stable, yet variable transfection of the construct in both peri-wound
tissue as well as rabbit dermal fibroblasts transfected in vitro. Optimisation of
this novel application in retroviral gene therapy could lead to effective anti-scarring
strategies.
Keywords
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Article info
Publication history
Published online: April 10, 2006
Accepted:
December 7,
2005
Received:
February 5,
2005
Footnotes
☆Presented at the 14th Annual Wound Healing Society Meeting, Atlanta, GA, 2004.
Identification
Copyright
© 2006 The British Association of Plastic Surgeons. Published by Elsevier Inc. All rights reserved.
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Access this article on ScienceDirectLinked Article
- Reduction of hypertrophic scar via retroviral delivery of a dominant negative TGF-beta receptor IIJournal of Plastic, Reconstructive & Aesthetic SurgeryVol. 60Issue 1
- PreviewHypertrophic scars (HSs) can cause significant aesthetic and functional symptoms and to-date no optimal treatment has been established. Many causes have been associated with HS development but regardless, it presents with appearance of myofibroblasts and an abnormal deposition of extracellular matrix (ECM) collagens. This accumulation makes ECM a logical target for pharmacological interventions, and researchers are endeavouring to modify ECM metabolism.
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