Advertisement

Osteogenesis of the construct combined BMSCs with β-TCP in rat

Published:December 17, 2008DOI:https://doi.org/10.1016/j.bjps.2008.11.017

      Summary

      Background

      The use of artificial bone graft substitutes has increased as the surgical applications widen and the availability of allograft bone decreases. The present study was to evaluate the construct combined bone marrow stromal cells (BMSCs) with β-tricalcium phosphate (β-TCP) as bone substitute implanted in rat dorsal muscles.

      Methods

      To study the osteogenic capability in vivo, specimens were harvested on 1 week, 4 weeks and 8 weeks after implantation, and were analyzed by hematoxylin and eosin (HE) staining. The percentages of new bone formation for each implant type and implantation period were determined by histomorphometry.

      Results

      After 1 week of implantation, new bone formation for both β-TCP and BMSCs+β-TCP group had no formed. After 4 weeks of implantation, the amount of bone formation was increased to 1.32 % in β-TCP group and 6.35% in BMSCs+β-TCP group. After 8 weeks of implantation, more bone was found in the BMSCs+β-TCP group (21.58 %), while in the β-TCP group bone formation was increased to 4.78%. Significant differences between the two groups have been observed.

      Conclusions

      Based on these results, we conclude that bone substitutes constructed by porous β-TCP scaffold loaded with osteogenically induced BMSCs could promote newly formed bone.

      Keywords

      To read this article in full you will need to make a payment

      References

        • Hirokazu K.
        • Takaaki T.
        • Masaaki C.
        • et al.
        Repair of segmental bone defects in rabbit tibiae using a complex of b-tricalcium phosphate, type I collagen, and fibroblast growth factor-2.
        Biomaterials. 2006; 27: 5118-5126
        • Khan S.N.
        • Tomin E.
        • Lane J.M.
        Clinical applications of bone graft substitutes.
        Orthop Clin North Am. 2000; 31: 389-398
        • Summers B.N.
        • Eisenstein S.M.
        Donor site pain from the ilium: a complication of lumbar spine fusion.
        J Bone Joint Surg Br. 1989; 7: 677-680
        • Friedlander G.E.
        Immune responses to osteochondral allografts: current knowledge and future directions.
        Clin Orthop. 1983; 174: 58-68
        • Langer R.
        • Vacanti J.P.
        Tissue engineering.
        Science. 1993; 260: 920-926
        • Anselme K.
        • Noel B.
        • Flautre B.
        • et al.
        Association of porous hydroxyapatite and bone marrow cells for bone regeneration.
        Bone. 1999; 25: 51S-54S
        • Cui Q.
        • Ming Xiao Z.
        • Balian G.
        • et al.
        Comparison of lumbar spine fusion using mixed and cloned marrow cells.
        Spine. 2001; 26: 2305-2310
        • Pittenger M.F.
        • Mackay A.M.
        • Beck S.C.
        • et al.
        Multilineage potential of adult human mesenchymal stem cells.
        Science. 1999; 284: 143-147
        • Muraglia A.
        • Martin I.
        • Cancedda R.
        • et al.
        A nude mouse model for human bone formation in unloaded conditions.
        Bone. 1998; 22: S131-S134
        • Arinzeh T.L.
        • Peter S.J.
        • Archambault M.P.
        • et al.
        Allogeneic mesenchymal stem cells regenerate bone in a critical-sized canine segmental defect.
        J Bone Joint Surg Am. 2003; 85A: 1927-1935
        • Quarto R.
        • Mastrogiacomo M.
        • Cancedda R.
        • et al.
        Repair of large bone defects with the use of autologous bone marrow stromal cells.
        N Engl J Med. 2001; 344: 385-386
        • Bruder S.P.
        • Jaiswal N.
        • Haynesworth S.E.
        Growth kinetics, self-renewal, and the osteogenic potential of purified human mesenchymal stem cells during extensive subcultivation and following cryopreservation.
        J Cell Biochem. 1997; 64: 278-294
        • Ogose A.
        • Hotta T.
        • Kawashima H.
        • et al.
        Comparison of hydroxyapatite and beta tricalcium phosphate as bone substitutes after excision of bone tumors.
        J Biomed Mater Res B Appl Biomater. 2005; 72: 94-101
        • Yuan J.
        • Cui L.
        • Zhang W.J.
        • et al.
        Repair of canine mandibular bone defects with bone marrow stromal cells and porous beta-tricalcium phosphate.
        Biomaterials. 2007; 28: 1005-1013
        • Stubbs D.
        • Deakin M.
        • Chapman-Sheath P.
        • et al.
        In vivo evaluation of resorbable bone graft substitutes in a rabbit tibial defect model.
        Biomaterials. 2004; 25: 5037-5044
        • Huffer W.E.
        • Benedict J.J.
        • Turner A.S.
        • et al.
        Repair of sheep long bone cortical defects filled with COLLOSS, COLLOSS E, OSSAPLAST, and fresh iliac crest autograft.
        J Biomed Mater Res B Appl Biomater. 2007; 82: 460-470
        • Naoki K.
        • Akira O.
        • Kunihiko T.
        • et al.
        Bone formation and resorption of hightly purified beta-tricalcium phosphate in the rat femoral condyle.
        Biomaterials. 2005; 26: 5600-5608
        • Engh C.A.
        • Hooten Jr., J.P.
        • Zettl Schaffer K.F.
        • et al.
        Evaluation of bone in-growth in proximally and extensively porous-coated anatomic medullary locking prostheses retrieved at autopsy.
        J Bone Joint Surg. 1995; 77A: 903-910
        • Lennon D.P.
        • Haynesworth S.E.
        • Young R.G.
        • et al.
        A chemically defined medium supports in vitro proliferation and maintains the osteochondral potential of rat marrow-derived mesenchymal stem cells.
        Exp Cell Res. 1995; 219: 211-222
        • Mendes S.C.
        • Tibbe J.M.
        • Veenhof M.
        • et al.
        Bone tissue-engineered implants using human bone marrow stromal cells: effect of culture conditions and donor age.
        Tissue Eng. 2002; 8: 911-920
        • Logeart-Avramoglou D.
        • Anagnostou F.
        • Bizios R.
        • et al.
        Engineering bone: challenges and obstacles.
        J Cell Mol Med. 2005; 9: 72-84
        • Lu J.X.
        • Flautre B.
        • Anselme K.
        • et al.
        Role of interconnections in porous bioceramics on bone recolonization in vitro and in vivo.
        J Mater Sci Mater Med. 1999; 10: 111-120
        • Habibovic P.
        • Yuan H.
        • van der Valk C.M.
        • et al.
        3D microenvironment as essential element for osteoinduction by biomaterials.
        Biomaterials. 2005; 26: 3565-3575
        • Karageorgiou V.
        • Kaplan D.
        Porosity of 3D biomaterial scaffolds and osteogenesis.
        Biomaterials. 2005; 26: 5474-5491
        • Hing K.A.
        • Annaz B.
        • Saeed S.
        • et al.
        Microporosity enhances bioactivity of synthetic bone graft substitutes.
        J Mater Sci Mater Med. 2005; 16: 467-475
        • Rickard D.J.
        • Sullivan T.A.
        • Shencker B.J.
        • et al.
        Induction of rapid osteoblast differentiation in rat bone marrow stromal cell cultures by dexamethasone and BMP-2.
        Dev Biol. 1994; 161: 218-228
        • Bi L.X.
        • Simmons D.J.
        • Mainous E.
        Expression of BMP-2 by rat bone marrow stromal cells in culture.
        Calcif Tissue Int. 1999; 64: 63-68
        • Frank O.
        • Heim M.
        • Jakob M.
        • et al.
        Real-time quantitative RT-PCR analysis of human bone marrow stromal cells during osteogenic differentiation in vitro.
        J Cell Biochem. 2002; 85: 737-746
        • Ripamonti U.
        • van den Heever B.
        • van Wyk J.
        Expression of the osteogenic phenotype in porous hydroxyapatite implanted extraskeletally in baboons.
        Matrix. 1993; 13: 491-502