Research Article| Volume 46, ISSUE 6, P460-465, 1993

Surface ultrastructure of human dermis and wounds

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      Scanning electron microscopic observations of the de-epidermalised surfaces of wounds and control skin reveal the architecture of dermal collagen. At wound edges there is a transition from the normal regular rete peg structure through erratically shaped finger-like projections with buds, to the relatively flat surface of granulation tissue. A corresponding change in the ultrastructure of dermal collagen is seen from normal areas where bundles of thick fibres are made up from finer fibrils, through a transitional zone where only fine fibrils are visible, to the granulation tissue where a disorganised amorphous structure is seen. It is suggested that the fibrous tissue surface at the edges of healing wounds is extensively remodelled after it is covered by epidermal cells.


      1. Clark RAF Henson PM The molecular and cellular biology of wound repair. Plenum Press, New York & London1988: 3-33
        • Stanley JR
        • Alvarez OM
        • Eaglestein WH
        • Katz SI
        Detection of basement zone antigens during epidermal wound healing in pigs.
        J Invest Dermatol. 1981; 79: 240-243
        • Compton CC
        • Gill JM
        • Bradford DA
        • Regauer S
        • Gallico GG
        • O'Connor NE
        Skin regenerated from cultured epithelial autografts on full thickness burn wounds from 6 days to 5 years after grafting. A light, electron microscopic and immunohistochemical study.
        Lab Invest. 1989; 60: 600-612
        • Moore JC
        • Shakespeare PG
        Morphology of human epidermal healing.
        J Tiss Viab. 1991; 1: 12-17
        • Kischer CW
        Collagen and mucopolysaccharides in the hypertrophic scar.
        Connect Tissue Res. 1974; 2: 205-213
        • Shakespeare PG
        • van Renterghem L
        Some observations on the surface structure of collagen in hypertrophic scars.
        Burns Inel Ther Inj. 1985; 11: 175-180
        • Oliver RF
        • Hulme MJ
        • Mudie A
        • et al.
        Skin collagen allografts in the rat.
        Nature. 1975; 258: 537-538
        • Walzer C
        • Benathan M
        • Frank E
        Thermolysin treatment: a new method for dermo-epidermal separation.
        J Invest Dermatol. 1989; 92: 78-81
        • Woodley D
        • Sauder D
        • Talley MJ
        • Silver M
        • Grotendorst G
        • Qwarnstrom E
        Localisation of basement membrane components after dermal-epidermal junction separation.
        J Invest Dermatol. 1983; 81: 149-153
        • Coons AH
        • Leduc EH
        • Connolly JM
        Studies on antibody production. I. A method for the histochemical demonstration of specific antibody and its application to a study of the hyperimmune rabbit.
        J Exp Med. 1955; 102: 49-59
        • Ryan GB
        • Majno G
        Upjohn Company, Kalamazoo. Michigan1977
        • Wagner RC
        Endothelial cell embryology and growth.
        Adv Microcirc. 1980; 9: 45-75
        • Burger PC
        • Klintworth GK
        Autoradiographic study of corneal neovascularisation induced by chemical cautery.
        Lab Invest. 1981; 45: 328-335
        • Briggaman RA
        • Wheeler CE
        The epidermal-dermal junction.
        J Invest Dermatol. 1975; 65: 71-84
        • Daroczy J
        • Feldman J
        • Kiraly K
        Human epidermal lamina: its structure, connections and function.
        Front Mat Biol. 1979; 7: 208-234
        • Stanley JR
        • Woodley DT
        • Katz SL
        • Martin GR
        Structure and function of basement membrane.
        J Invest Dermatol. 1982; 79 (suppl): 69-72