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Research Article| Volume 46, ISSUE 2, P136-142, January 1993

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Role and innocuity of Tisseel®, a tissue glue, in the grafting process andin vivo evolution of human cultured epidermis

  • F.A. Auger
    Correspondence
    Requests for repritns to: Dr F. Auger at the Laboratoire de Recherche des Grands Bruˆle´s/LOEX.
    Affiliations
    Laboratoire de Recherche des Grands Brlˆe´s/LOEX, Hoˆpital du Saint-Sacrement, 1050 Chemin Sainte-Foy, Que´bec (Que´bec, Canada G1S 4L8

    De´partement de Chirurgie, Universite´Laval, Faculte´de Me´decine, Sainte-Foy, Que´bec, Canada
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  • Rina Guignard
    Affiliations
    Laboratoire de Recherche des Grands Brlˆe´s/LOEX, Hoˆpital du Saint-Sacrement, 1050 Chemin Sainte-Foy, Que´bec (Que´bec, Canada G1S 4L8
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  • Carlos A. Lo´pez Valle
    Affiliations
    Laboratoire de Recherche des Grands Brlˆe´s/LOEX, Hoˆpital du Saint-Sacrement, 1050 Chemin Sainte-Foy, Que´bec (Que´bec, Canada G1S 4L8

    De´partement de Chirurgie, Universite´Laval, Faculte´de Me´decine, Sainte-Foy, Que´bec, Canada
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  • Lucie Germain
    Affiliations
    Laboratoire de Recherche des Grands Brlˆe´s/LOEX, Hoˆpital du Saint-Sacrement, 1050 Chemin Sainte-Foy, Que´bec (Que´bec, Canada G1S 4L8

    De´partement de Chirurgie, Universite´Laval, Faculte´de Me´decine, Sainte-Foy, Que´bec, Canada
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      Abstract

      Cultured epidermal sheets are currently used for burn wound treatment but reported results on graft take are variable. This study was designed to evaluate the role and influence of Tisseel®, a fibrin glue, in the take of cultured human epidermal sheets in an athymic mouse model. On days 4, 10 and 21 post-grafting, histology, electron microscopy and immunofluorescence staining confirmed the presence of a human epithelium and the development of a basement membrane. Tisseel® was detectable on day 4 only, but overall treated and untreated grafts were similar. The use of Tisseel® enhanced the mechanical stability of these fragile grafts, increased the percentage of graft take, and its innocuity on thein vivo evolution of cultured epidermal sheets was demonstrated. For these reasons, we think that Tisseel® may be advantageous in a clinical setting.

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