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Abstract
44 PTFE prostheses (Gore-Tex®; ID 1 mm) were implanted into rats' femoral veins by means of the sleeve anastomotic technique and were evaluated at regular intervals from 1 h up till 24 weeks after implantation by means of light and electron microscopy to study in detail their healing process.
All prostheses, except one at 1 week and one at 24 weeks after implantation, were patent at the time of removal. Upon implantation, the luminal surface of the prostheses became covered with a thin clot layer. From 1 week onwards, endothelial cells originating from the anastomotic sides grew in across the anastomoses. In addition, small capillarylike orifices were present at the anastomotic sites, from which endothelial cells also seemed to originate. At 2 weeks, in several areas in the mid-region of the prostheses, the fronts of regenerating endothelial cells had reached each other, and about 80% of the luminal surface was covered by endothelium and at 3 weeks, the prostheses were completely covered by an endothelial layer.
These results demonstrate that PTFE microvenous prostheses heal exclusively by means of rapid ingrowth of endothelial cells originating from both sides at the anastomoses.
Reference
- Multipotential cells in the circulating blood: ultrastructural evidence in the calf.Transactions of the American Society of Artificial Internal Organs. 1982; 28: 185
- Microvascular synthetic grafts.Plastic and Reconstructive Surgery. 1980; 66: 380
- Repair in arterial tissue. Demonstration of fibrogen/fibrin in the normal and healing rabbit thoracic aorta by the indirect immunoperoxidase technique.Virchows Archives of Pathology and Anatomy. 1984; 403: 163
- Mechanisms of arterial graft healing.American Journal of Pathology. 1986; 123: 220
- Patency of synthetic microvenous conduits: an experimental study in the femoral vein of the rat.Plastic and Reconstructive Surgery. 1986; 78: 378
- Polytetra-fluoroethylene reconstruction of the inferior vena cava.Surgery. 1984; 95: 625
- The use of prostacyclin analogue-containing suture for the prevention of postoperative venous thrombosis in the rat.Plastic and Reconstructive Surgery. 1986; 78: 504
- Effects of serum lipoproteins on the morphology, growth and metabolism of arterial smooth muscle cells.Advances in Experimental Medicine and Biology. 1974; 43: 299
- Polytetrafluoroethylene grafts in the peripheral venous circulation of rabbits.American Journal of Surgery. 1983; 146: 355
- Comparison of expanded polytetrafluoroethylene microvascular grafts to autogenous vein grafts.Plastic and Reconstructive Surgery. 1982; 70: 193
- Pseudoendothelium formation in expanded polytetrafluoroethylene grafts implanted in the inferior vena cava of normal and defibrinogated dogs.Artificial Organs. 1981; 5: 379
- New observations on the healing process in prosthetic substitution of large veins by microporous graft. Animal experiments.Thoracic and Cardiovascular Surgeon. 1989; 37: 119
- Experimental studies on absorbable vascular grafts for microsurgery.Scandinavian Journal of Plastic and Reconstructive Surgery. 1983; 17: 133
- Expanded polytetrafluoroethylene as a microvascular graft: an experimental study.Journal of Microsurgery. 1980; 1: 447
- Experimental microvascular polytetrafluoroethylene grafts: 6-month patency.Plastic and Reconstructive Surgery. 1985; 76: 748
- Experimental evaluation of autogenous and prosthetic vein grafts in microsurgery.International Journal of Microsurgery. 1979; 1: 36
- Prosthetic microvenous grafting into the femoral vein of the rat. Considerations concerning the anastomotic technique.British Journal of Plastic Surgery. 1989; 42: 538
- Patency and healing of polymeric microvenous prostheses implanted into the rat femoral vein by means of the sleeve anastomotic technique.Journal of Reconstructive Microsurgery. 1990; 6: 287
- A platelet dependent serum factor that stimulates the proliferation of arterial smooth muscle cells in vitro.in: Proceedings of the National Academy of Science. 71. 1974: 1207
- The use of long synthetic microvascular grafts to vascularise free flaps in rabbits.British Journal of Plastic Surgery. 1988; 41: 305
- Endothelium grown for circulating blood on isolated intravascular dacron hub.American Journal of Pathology. 1963; 43: 361
- Patency rates in microvascular prosthesis: an experimental study.British Journal of Plastic Surgery. 1981; 34: 72
- Microvascular polytetrafluoroethylene prostheses: the cellular events of healing and prostacyclin production.Plastic and Reconstructive Surgery. 1988; 81: 735
- Improved healing of microvascular PTFE prostheses by induction of a clot layer. An experimental study in rats.Plastic and Reconstructive Surgery. 1989; 84: 960
- Expanded polytetrafluoroethylene patch for the repair of large abdominal wall defects.British Journal of Surgery. 1989; 76: 803
- Experimental microvenous reconstructions with Gore-Tex polytetrafluoroethylene prostheses implanted by means of the sleeve anastomotic technique.Microsurgery. 1991; 12: 23
- Enhanced healing of 30 μm Gore-Tex microarterial prostheses by alcohol pretreatment.British Journal of Plastic Surgery. 1991; 44: 428
Article info
Publication history
Accepted:
September 7,
1992
Received:
July 28,
1992
Identification
Copyright
© 1993 The British Association of Plastic Surgeons. Published by Elsevier Inc. All rights reserved.