Research Article| Volume 46, ISSUE 7, P565-569, 1993

Mechanical properties of skin graft wounds

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      In female Wistar rats the mechanical strength development of the wound between a skin graft and the neighbouring intact skin (graft wound) was compared with that of ordinary incisional wounds after 4, 7, 14 and 21 days of healing. In one group of rats a 35 × 20 mm skin graft including the subcutaneous muscle was raised and replacedin situ on the left side of the back and a 35 mm incisional wound was made on the right side. In another group a 35 mm incisional wound was made on the right side of the back only. After 4 days the maximum load, maximum stiffness and relative failure energy of the graft wounds were 49, 43 and 40% less respectively than those of the incisional wounds from the same animals and after 7 days the maximum load and maximum stiffness of the graft wounds were reduced by 26 and 29%. However, after 14 and 21 days no differences in mechanical properties were found between these two types of wounds. Compared with the incisional wound from rats without graft the maximum load, maximum stiffness and relative failure energy of the graft wound were reduced by 57, 58 and 44% after 4 days, 59, 62 and 54% after 7 days, 37, 38 and 29% after 14 days and for maximum load and maximum stiffness a reduction of 33 and 31% was found after 21 days of healing.


        • Marckmann A
        Reaction of skin to autotransplantation.
        in: Studies on the microcirculation and the biochemical composition in dermis. Munksgaard, Copenhagen1966: 112
        • Klein K
        • Rudolph R
        3H-collagen turnover in skin grafts.
        Surg Gynecol Obstet. 1972; 135: 49-57
        • Zika JM
        • Rudolph R
        • Klein L
        Autoradiographic distribution of collagen loss in skin grafts.
        Anat Rec. 1973; 177: 377-392
        • Tavis MJ
        • Thornton JW
        • Harney JH
        • Danet RT
        • Woodroof EA
        • Bartlett RH
        Mechanism of skin graft adherence: collagen, elastin and fibrin interactions.
        in: Surgical Forum. 28. 1977: 522-524
        • Arnold F
        • West D
        • Kumar S
        Wound healing: the effect of macrophage and tumour derived angiogenesis factors on skin graft vascularization.
        Br J Exp Path. 1987; 68: 569-574
        • Viljanto J
        Biochemical basis of tensile strength in wound healing.
        Acta Chir Scand. 1964; : 111
        • Doillon CD
        • Dunn MG
        • Bender E
        • Silver FH
        Collagen fiber formation in repair tissue: development of strength and toughness.
        Collagen and Related Research. 1985; 5: 481-492
        • Ross R
        • Benditt EP
        Wound healing and collagen formation. Sequential changes in components of guinea pig skin wounds observed in the electron microscope.
        J Biophys Biochem Cytology. 1961; 11: 677-700
        • Scharfetter K
        • Kulozik M
        • Stolz W
        • et al.
        Localization of collagen α1(I) gene expression during wound healing by in situ hybridization.
        J Invest Dermatol. 1989; 93: 405-412
        • Jørgensen PH
        • Jensen KH
        • Andreassen TT
        Mechanical strength in rat skin incisional wounds treated with fibrin sealant.
        J Surg Res. 1987; 42: 237-241
        • Jørgensen PH
        • Andreassen TT
        Influence of biosynthetic human growth hormone on biomechanical properties of rat skin incisional wounds.
        Acta Chir Scand. 1988; 154: 623-626
        • Oxlund H
        Relationships between the biomechanical properties, composition and molecular structure of connective tissues.
        Connect Tissue Res. 1986; 15: 65-72
        • Delany HM
        • Demetriou AA
        • Teh E
        • Levenson SM
        Effect of early postoperative nutritional support on skin wound and colon anastomosis healing.
        J Parent Enteral Nutr. 1990; 14: 357-361
        • Peacock EE
        Effect of dietary proline and hydroxyproline on tensile strength of healing wounds.
        in: Proceedings of the Society for Experimental Biology and Medicine. 105. 1960: 380-383
        • Fogdestam I
        A biomechanical study of healing rat skin incisions after delayed primary closure.
        Surg Gynecol Obstet. 1981; 153: 191-199