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Abstract
In wound healing research, animal models permit an extensive tissue analysis which is not normally possible in clinical studies. A morphological comparison of human and porcine skin was made in order to identify those aspects of the wound healing process where a porcine model may help our understanding of clinical problems. We describe a porcine model for evaluating the growth of cultured keratinocytes on a variety of wound beds. Polytetrafluoroethylene skin graft chambers were used to isolate wounds and prevent epidermal healing from the skin edge. The chambers remainedin situ for 5–7 weeks. We detail the surgical technique, the method of porcine keratinocyte culture and highlight some practical measures that were taken to optimise the “take” of the cultured keratinocyte grafts.
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Article info
Publication history
Accepted:
February 11,
1993
Received:
January 4,
1993
Footnotes
The work was supported by the Restoration of Appearance and Function Trust (RAFT).
Identification
Copyright
© 1993 The British Association of Plastic Surgeons. Published by Elsevier Inc.