This paper is only available as a PDF. To read, Please Download here.
Abstract
The attachment of grafts of keratinocyte sheets is mediated in part by the presence and organisation of basement membrane components. The reappearance of basement membrane following keratinocyte autografting was examined in pigs. These studies showed that there was rapid expression of anchoring fibrils and hemidesmosomes, which reached normal numbers at 10 days. However, the length of hemidesmosomes did not reach normal size during the period of study. Weakness of attachment of keratinocyte autografted epidermis was found to lie between the basement membrane and the granulation tissue. This suggests that reported clinical problems with keratinocyte graft attachment may be mediated not only by delay in maturation of the basement membrane but also by its poor integration with collagen of the wound bed.
Reference
- New grafts for old? A review of alternatives to autologous skin.Br J Plast Surg. 1992; 45: 354-363
- Grafting of burns with cultured epithelium prepared from autologous epidermal cells.Lancet. 1981; 1: 75-78
- Treatment of chronic venous ulcers with sheets of cultured allogenic keratinocytes.Br J Dermatol. 1987; 117: 591-597
- Composite autologous-allogeneic skin replacement: development and clinical application.Plast Reconstr Surg. 1987; 80: 626-635
- Reconstitution of structure and cell function in human skin grafts derived from cryopreserved allogeneic dermis and autologous cultured keratinocytes.J Invest Dermatol. 1988; 91: 478-485
- Skin regenerated from cultured epithelial autografts on full-thickness burn wounds from 6 days to 5 years after grafting.Lab Invest. 1989; 60: 600-612
- Burn wounds resurfaced by cultured epidermal autografts show abnormal reconstitution of anchoring fibrils.JAMA. 1988; 259: 2566-2571
- Cultured epithelial autografts for giant congenital naevi.Plast Reconstr Surg. 1989; 84: 1-9
- Transplantation studies on sheets of pure epidermal epithelium and on epidermal cell suspensions.Br J Plast Surg. 1952; 5: 25-36
- The basement membrane.Arch Derm. 1988; 124: 709-712
- The surgical management of Dystrophic Epidermolysis Bullosa (excluding the hand).Br J Plast Surg. 1992; 45: 426-434
- Epithelial-mesenchymal interactions enhance expression of collagen VII in vitro.J Invest Dermatol. 1991; 96: 803-808
- Healing of full-thickness cutaneous wounds in the pig. I. Immunohistochemical study of epidermo-dermal junction regeneration.J Invest Dermatol. 1991; 96: 777-785
- Ontogenesis of the basement membrane zone after grafting cultured human epithelium.J Invest Dermatol. 1992; 99: 71-77
- Ultrastructural morphometry of normal human dermal-epidermal junction. The influence of age, sex and body region on laminar and non-laminar components.J Invest Dermatol. 1984; 83: 448-453
- Hemidesmosome heterogeneity in junctional epidermolysis bullosa revealed by morphometric analysis.J Invest Dermatol. 1986; 86: 51-56
- The effect of dressing materials on keratinocyte autograft take.Br J Plast Surg. 1993; 46: 228-234
- Hemidesmosome and desmosome morphogenesis during epidermal wound healing.J Ultratruct Res. 1973; 45: 93-101
- Growth and differentiation of human epidermal cultures used as auto- and allografts in humans.Br J Dermatol. 1987; 116: 161-170
- Clinical, histological and ultrastructural aspects of cultured epithelium.in: Teepe RGC Proceedings of the International Symposium on the clinical use of cultured epithelium in surgery and dermatology, Leiden Medical and Scientific Conferences Ltd, The Netherlands. Wheathampstead, EnglandMarch 1987: 36-45
- Ultrastructural study of grafted autologous cultured human epithelium.Br J Plast Surg. 1989; 42: 35-42
- Wound coverage with cultured autologous keratinocytes: use after burn wound excision, including biopsy followup.J Trauma. 1988; 28: 195-198
- Formation and origin of basal lamina and anchoring fibrils in adult human skin.J Cell Biol. 1971; 51: 384-395
- Epidermolysis bullosa acquisita antigen is synthesized by both human keratinocytes and human dermal fibroblasts.J Invest Dermatol. 1985; 85: 542-545
- Methods of cultivation of keratinocytes with an air liquid interface.J Invest Dermatol. 1983; 81: 28s-33s
- Characterization of “neo-dermis” formation beneath cultured human epidermal autografts transplanted on muscle fascia.J Invest Dermatol. 1990; 95: 20-26
- Composite skin grafts: frozen dermal allografts support the engraftment and expansion of autologous epidermis.J Trauma. 1985; 25: 106-112
- Hemidesmosome formation in vitro.J Cell Biol. 1983; 97: 849-857
Article info
Publication history
Accepted:
March 1,
1993
Received:
December 11,
1992
Identification
Copyright
© 1993 The British Association of Plastic Surgeons. Published by Elsevier Inc.
