Journal of Plastic, Reconstructive & Aesthetic Surgery
Volume 60, Issue 8 , Pages 864-875 , August 2007

Perivascular cells in a skin graft are rapidly repopulated by host cells

  • S. O'Ceallaigh

      Affiliations

    • UK Centre for Tissue Engineering, Faculty of Life Sciences, UK
    • ,
  • S.E. Herrick

      Affiliations

    • UK Centre for Tissue Engineering, Faculty of Life Sciences, UK
    • ,
  • W.R. Bennett

      Affiliations

    • UK Centre for Tissue Engineering, Faculty of Life Sciences, UK
    • ,
  • J.E. Bluff

      Affiliations

    • UK Centre for Tissue Engineering, Faculty of Life Sciences, UK
    • ,
  • M.W.J. Ferguson

      Affiliations

    • UK Centre for Tissue Engineering, Faculty of Life Sciences, UK
    • ,
  • D.A. McGrouther

      Affiliations

    • Plastic and Reconstructive Surgery Research, University of Manchester, Room 3.106 Stopford Building, Oxford Road, Manchester M13 9PT, UK
    • Corresponding Author InformationCorresponding author. Tel.: +441612751591; fax: +441612751813.

Received 16 January 2006 ,Accepted 5 March 2006.

References 

  1. O'Ceallaigh S, Herrick SE, Bluff JE, et al. Quantification of total and perfused blood vessels in murine skin autografts using a fluorescent double-labeling technique. Plast Reconstr Surg. 2006;117(1):140–151
  2. McCluskey J, Martin P. Analysis of the tissue movements of embryonic wound healing – DiI studies in the limb bud stage mouse embryo. Dev Biol. 1995;170(1):102–114
  3. Okabe M, Ikawa M, Kominami K, et al. ‘Green mice’ as a source of ubiquitous green cells. FEBS Lett. 1997;407(3):313–319
  4. Patterson GH, Knobel SM, Sharif WD, et al. Use of the green fluorescent protein and its mutants in quantitative fluorescence microscopy. Biophys J. 1997;73(5):2782–2790
  5. Boyce ST, Goretsky MJ, Greenhalgh DG, et al. Comparative assessment of cultured skin substitutes and native skin autograft for treatment of full-thickness burns. Ann Surg. 1995;222(6):743–752
  6. Braverman IM, Yen A. Ultrastructure of the human dermal microcirculation. II. The capillary loops of the dermal papillae. J Invest Dermatol. 1977;68(1):44–52
  7. Braverman IM. Ultrastructure and organization of the cutaneous microvasculature in normal and pathologic states. J Invest Dermatol. 1989;93(Suppl. 2):2S–9S
  8. Clemmesen T. The early circulation in split skin grafts: restoration of blood supply to split-skin grafts. Acta Chir Scand. 1964;127:1–8
  9. Stevens SM. The restoration of the vasculature of skin autografts in the rabbit. Pathology. 1975;7(2):79–90
  10. Birch J, Branemark PI, Lundskog J. The vascularization of a free full thickness skin graft. II. A microangiographic study. Scand J Plast Reconstr Surg. 1969;3(1):11–17
  11. Haller JA, Billingham RE. Studies of the origin of the vasculature in free skin grafts. Ann Surg. 1967;166(6):896–901
  12. Marckmann A. Autologous skin grafts in the rat: vital microscopic studies of the microcirculation. Angiology. 1966;17(7):475–482
  13. Goretsky MJ, Breeden M, Pisarski G, et al. Capillary morphogenesis during healing of full-thickness skin grafts: an ultrastructural study. Wound Repair Regen. 1995;3:213–220
  14. Zarem HA, Zweifach BW, McGehee JM. Development of microcirculation in full thickness autogenous skin grafts in mice. Am J Physiol. 1967;212(5):1081–1085
  15. Plenat F, Vignaud JM, Guerret-Stocker S, et al. Host–donor interactions in healing of human split-thickness skin grafts onto nude mice: in situ hybridization, immunohistochemical, and histochemical studies. Transplantation. 1992;53(5):1002–1010
  16. Oswald J, Boxberger S, Jorgensen B, et al. Mesenchymal stem cells can be differentiated into endothelial cells in vitro. Stem Cells. 2004;22(3):377–384
  17. Lin Y, Weisdorf DJ, Solovey A, et al. Origins of circulating endothelial cells and endothelial outgrowth from blood. J Clin Invest. 2000;105(1):71–77
  18. Wang HJ, Wan HL, Yang TS, et al. Acceleration of skin graft healing by growth factors. Burns. 1996;22(1):10–14
  19. Rinsch C, Quinodoz P, Pittet B, et al. Delivery of FGF-2 but not VEGF by encapsulated genetically engineered myoblasts improves survival and vascularization in a model of acute skin flap ischemia. Gene Ther. 2001;8(7):523–533
  20. Zhang F, Oswald T, Lin S, et al. Vascular endothelial growth factor (VEGF) expression and the effect of exogenous VEGF on survival of a random flap in the rat. Br J Plast Surg. 2003;56(7):653–659
  21. Lees VC, Fan TP. A freeze-injured skin graft model for the quantitative study of basic fibroblast growth factor and other promoters of angiogenesis in wound healing. Br J Plast Surg. 1994;47(5):349–359
  22. Gruss CJ, Satyamoorthy K, Berking C, et al. Stroma formation and angiogenesis by overexpression of growth factors, cytokines, and proteolytic enzymes in human skin grafted to SCID mice. J Invest Dermatol. 2003;120(4):683–692
  23. Khozani TT, Noorafshan A, Nikeghbalian S, et al. Stereologic study of the effects of prostaglandin E2 on the induction of angiogenesis in full-thickness skin autografts. Adv Skin Wound Care. 2004;17(4 Pt 1):202–204[206]

☆ This work was presented at the Winter Meeting of the British Association of Plastic Surgeons, at the Royal College of Surgeons in London, December 2004.

PII: S1748-6815(06)00283-X

doi: 10.1016/j.bjps.2006.03.036

Journal of Plastic, Reconstructive & Aesthetic Surgery
Volume 60, Issue 8 , Pages 864-875 , August 2007

Article Tools

* Image Usage & Resolution